1011: SULFURIMONAS MJ MEDIUM
Modifications: Omit thiosulfate and supplement medium with 4.00 g/l yeast extract and 4.00 g/l Trypton peptone. After autoclaving the medium is reduced with 0.30 g/l Na2S x 9 H2O added from a sterile anoxic stock solution (3% w/v) prepared under 100% N2 gas and the pH adjusted to 6.5. After inoculation pressurize vessels to 1 bar overpressure with sterile 80% N2 and 20% CO2 gas mixture. Do not add sterile air!
Growth observed:
Growth conditions: 2-3 days anaerobic 55 °C